Lipase (Candida) / Lipase / Lipolytic Enzyme / Fat-Splitting Enzyme / Triacylglycerol Acylhydrolase / Microbial Lipase / Lipase (Candida)

English Name: Lipase (Candida); Lipase from Candida; Microbial lipase
Other Names: Lipase; Fat hydrolytic enzyme; Fat-decomposing enzyme; Triacylglycerol acylhydrolase; Microbial lipase
CAS Number: 9001-62-1

Grade: BR
Molecular Weight: Generally 67,000
Activity: ≥5000 u/g
Loss on Drying: ≤8.0%

Temperature Stability: Stable below 40°C, severely inactivated above 50°C. Short-term use at 40–42°C is suitable; long-term use at 38–40°C is recommended.

pH Stability: Optimal at pH 7.0–7.5
Appearance: Gray-brown or light gray powder. Microbial strain: Candida lipolytica. Belongs to the class of fat hydrolyzing enzymes, alongside proteases and amylases as the three major hydrolytic enzymes. Optimal temperature: 35–37°C. Enzyme reaction: Triglyceride + H₂O = Diglyceride + Fatty acid anion. Most easily hydrolyzes primary ester groups. Monoglycerides are poor substrates. Two nearly identical isozyme lipases: A and B. Isoelectric points (pI): A is 4.9, B is 5.0. Extinction coefficient (E1%280): 13.3. Activator: Calcium (Ca²⁺).

Use: Biochemical research. An enzyme that catalyzes the hydrolysis of fats. Industrially used for fat hydrolysis to produce fatty acids, leather degreasing, detergent industry, etc.
Storage: 2–8°C